专利名称：METHODS AND SYSTEMS FOR ESTIMATING
THE MELTING TEMPERATURE (Tm) FORPOLYNUCLEOTIDE MOLECULES
发明人：OWCZARZY, Richard,WALDER, Joseph,
Alan,HUANG, Lingyan,BEHLKE, Mark, Aaron
申请号：EP03754518.3申请日：20030912公开号：EP1543438B1公开日：20090506
摘要：The invention relates to methods and systems for predicting or estimating themelting temperature of duplex nucleic acids, particularly duplexes of oligonucleotideswhich may be used, for example, as primers or probes in PCR and/or hybridization assays.The invention also relates to methods and systems for designing and selectingoligonucleotide probes and primers having a predicted melting temperature which isoptimized for such assays. To this end, algorithms and methods are provided forpredicting the melting temperature of a nucleic acid having a predetermined sequence.These methods and algorithms estimate the melting temperature of a nucleic acidduplex under particular salt conditions. The methods and algorithms use novel formulas,having terms and coefficients that are functions of the particular nucleotide sequence, toestimate the effect of particular salt conditions on the melting temperature. As such, themethods and systems of the invention provide superior result compared to existingmethods, which do not consider sequence dependent effects of changing salt conditions.
申请人：INTEGRATED DNA TECH INC
地址：US
国籍：US
代理机构：Howard, Paul Nicholas
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