Method for producing cytoplasmic male sterility in

专利名称：Method for producing cytoplasmic male

sterility in plants and use thereof inproduction of hybrid seed

发明人：Pal Maliga申请号：US08/217360申请日：19940324公开号：US05530191A公开日：19960625

摘要：The present invention provides a cytoplasmic male sterility (CMS) system forplants, which is based on modification of the plastid genome. The CMS system comprisesthree transgenes: a \"plastid male sterility\" (pms) gene, regulated by a \"nuclear malesterility\" (rims) gene and a \"restorer of male fertility\" (rmf) gene. The pms gene is eitheran inactive gene encoding a toxic gene product or an actively expressed gene encodingan essential gene product. The runs gene is controlled by an anther-specific promoter,and encodes a plastid-directed polypeptide that regulates the pms gene, either byactivating expression of the inactive toxic pms gene or by repressing expression of theessential pms gene, which causes plastid and cellular disablement or death in anthertissue, thereby preventing formation of viable pollen. The rmf gene encodes a geneproduct that prevents the rims gene from regulating the pms gene. Cytoplasmicallymale-sterile plants are obtained by crossing a parent line containing the pms gene with aparent line containing the nms gene. Male-fertile hybrid seed is obtained from crossesbetween the cytoplasmically male-sterile plants and a second parent line containing thermf gene.

申请人：RUTGERS, THE STATE UNIVERSITY OF NEW JERSEY

代理人：Janet E.Dann, Dorfman, Herrell and Skillman Reed

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