MICROORGANISMS AND METHODS FOR CARBON-EFFICIENT BI

专利名称：MICROORGANISMS AND METHODS FOR

CARBON-EFFICIENT BIOSYNTHESIS OF MEKAND 2-BUTANOL

发明人：OSTERHOUT, ROBIN, E.,NIU, WEI,BURGARD,

ANTHONY, P.

申请号：EP10786874申请日：20100610公开号：EP2440669A4公开日：20130828

摘要：A non-naturally occurring microbial organism has at least one exogenousnucleic acid encoding a MEK pathway enzyme expressed in a sufficient amount to produceMEK. The MEK pathway includes an enzyme selected from an acetoacetyl-CoAdehydrogenase (bifunctional), an acetoacetyl-CoA aldehyde dehydrogenase, a 3-oxobutyraldehyde reductase, a 3-oxobutanol dehydratase, an MEK oxidoreductase, a 3-oxobutyraldehyde aminotransferase, a 4-aminobutan-2-one deaminase, a 2-amino-4-ketopentanoate (AKP) thiolase, an AKP aminotransferase, a 2,4-dioxopentanoatedecarboxylase, an AKP deaminase, an acetylacrylate decarboxylase, an AKP

decarboxylase, a glutamate dehydrogenase, a 3-oxobutyraldehyde oxidoreductase(aminating) and an AKP oxidoreductase (aminating). A 2-butanol pathway further includesan MEK reductase. A method for producing MEK or 2-butanol includes culturing theseorganisms under conditions and for a sufficient period of time to produce MEK or 2-butanol.

申请人：GENOMATICA, INC.

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